Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc MES Free Acid 250 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Electron Microscopy Sciences D-PBS, Formula I Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set crystals | 100g
Trizma Pre-set crystals | MW: 151.6 (average) | 100g
Abcam E x traction Buffer 5 x PTR, 50ML
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types
The product is subject to the following: Abcam Restricted Use Statement
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Teknova 5M Sodium Chloride Solution, 200L Bag
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Electron Microscopy Sciences Hank's Balanced Salts Formula II 1 LT
All of our Hanks' Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.
STEMCELL Technologies RoboSep™ Buffer (5X Concentrate), Size: 250 mL
RoboSep™ Buffer is recommended for EasySep™ cell separation protocols performed by RoboSep™ . Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Sigma Aldrich Fine Chemicals Biosciences TRIS BUFFERED SALINE, WITH BSA
Tris Buffered Saline, with BSA, pH 8.0 Powder
Teknova 1X PBS Solution, pH 7.4, 200L Bag, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Gold Biotechnology Inc MOPS Free Acid Ultra Pure
MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 6 5 7 9 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Teknova 10X PBS-MK, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 25 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C
Teknova 1M Tris-HCl, pH 8.3, 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.